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CLOUD-CLONE Corp.USA
 
 
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ELISA Kit for C Reactive Protein (CRP)--$99.0 per 96T 

  产品分类 : 生化科技->醫療器材->酵素免疫試劑

     
ELISA Kit for C Reactive Protein (CRP)--$99.0 per 96T
 
         

  产品规格
 
Model No:    SEA821Hu 96T
  Country  0
  Brand  CLOUD-CLONE
  Materials  reagent
  Size  96T    
  Weight  3kg
  Color  blue
  Packing  96T
  Minimum Order  1
  Payment Term  pre-payment

 

  产品描述
   
  ELISA Kit for C Reactive Protein (CRP)
C-RP; PTX1; Pentraxin-Related; Pentraxin 1
Specificity
This assay has high sensitivity and excellent specificity for detection of C Reactive Protein (CRP).
No significant cross-reactivity or interference between C Reactive Protein (CRP) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant C Reactive Protein (CRP) and the recovery rates were calculated by comparing the measured value to the expected amount of C Reactive Protein (CRP) in samples.
Matrix Recovery range (%) Average(%)
serum(n=5) 90-104 94
EDTA plasma(n=5) 89-97 92
heparin plasma(n=5) 82-96 91
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C Reactive Protein (CRP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C Reactive Protein (CRP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C Reactive Protein (CRP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-89% 79-98% 98-105% 99-105%
EDTA plasma(n=5) 82-101% 89-98% 93-101% 94-101%
heparin plasma(n=5) 98-105% 93-102% 92-99% 91-98%
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C Reactive Protein (CRP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C Reactive Protein (CRP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C Reactive Protein (CRP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C Reactive Protein (CRP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

   


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